pco.flim相機系統(tǒng)是采用Two-Tap CMOS成像芯片的熒光壽命成像相機。相機的像素和激發(fā)光間的同步使100皮秒至100微秒間的熒光衰減時間的分析成為可能。其多種觸發(fā)選項使相機可以方便的集成于多種實際應(yīng)用。

德國PCO公司首次發(fā)布了這款采用Two-Tap CMOS技術(shù)的熒光壽命成像系統(tǒng)Flim，應(yīng)用范圍涵蓋分子特性分析和測試，F(xiàn)RET，離子濃度/PH值測量，生物組織學(xué)/病理學(xué)，壓感材料等，可廣泛應(yīng)用于生命科學(xué)、物理科學(xué)，材料科學(xué)領(lǐng)域。

主要特點：

• 100 ps到100μs的壽命測量

• 調(diào)制頻率從5千赫茲到40兆赫

• 500千赫茲到40兆赫外部調(diào)制信號

• 正弦/矩形的調(diào)制信號波形

• 像素分辨率1008 x 1008

• 頻域FLIM

• USB 3.0接口

• 量子效率39%

• 動態(tài)范圍1000:1

• 讀出噪音45 e- rms

• 90幀每秒 (2 tap讀出)

• 可選曝光時間為10ns到10秒

• 無振動水冷

• 特殊的測量與分析軟件

技術(shù)參數(shù)：

應(yīng)用案例：

Figure 1: The left photo shows the fluorescence intensity of HEK-293 cells which expressed a CFP/DJ-1 protein as control of a FRET experiment. The middle image shows the phase angle derived distribution of fluorescence lifetimes in the range of 0 – 4 ns (imageJ LUT 16 colors, colorbar 0 – 4 ns) which has been masked by an intensity filter. The range of lifetimes around 2 ns was found in all of the 26 cells, which have been measured and showed about 10% FRET efficiency compared to the pure CFP expression. The right image shows the lifetime distribution image weighted by the fluorescence intensity image (the color images are false color coded using the same colorbar and LUT) without mask (courtesy of Prof. Dr. F.S. Wouters and Dr. G. Bunt, University Medicine G?ttingen).

Figure 5:The left image shows the fluorescence intensity of a sliced daisy sample (20x air objective). The middle image shows the phase angle derived distribution of fluorescence lifetimes in the range of 0 - 4 ns (imageJ LUT 16 colors, colorbar 0-4 ns). The difference in fluorescence lifetimes clearly allows to differentiate the cells and the pollen grains. The right image shows the lifetime distribution image weighted by the fluorescence intensity image (the color images are false color coded, the modulation frequency was 30 MHz) (courtesy of Prof. Dr. F.S. Wouters and Dr. G. Bunt, University Medicine G?ttingen).

注：產(chǎn)品信息若有變更恕不另行通知